Studies of various microbial contaminants during culturing of amniotic fluid derived cells in buffalo (Bubalus bubalis)

Abstract

Cell culture complexity is as shared as that of culture failure due to contamination. All cell culture in laboratories and cell culture workers have acknowledged it. Culture contaminants may be organic or elementary, seen or hidden, unhelpful or apparently gentle, but in all gear they harmfully influence both the use of cell cultures and the excellence of research work. Major microbial contaminants in the cell culture include Staphylococcus epidermidis, S. aureus, Bacillus subtilis, Escherichia. coli, Mycoplasma orale, M. fermentans and Saccharomyces cerevisiae. There are several factors which influence the amniotic fluid (AF) cell culture like level of blood cells, volume of amniotic fluid, attachment time, number and microbial contamination. Amniotic fluid derived cells in buffalo (Bubalus bubalis) were cultured in normal suitable media and effects of microbial contaminants were studied.